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compound 48 80 solution  (Valiant Co Ltd)

 
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    Valiant Co Ltd compound 48 80 solution
    Compound 48 80 Solution, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/compound+48+80+solution/Compound+48%2F80/pm28458347-53-16-19
    Average 90 stars, based on 11 article reviews
    compound 48 80 solution - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand.
    Article Snippet: Hyaluronidase Activity Inhibitory Assay The hyaluronidase activity inhibitory assay was performed using the method of Fujitani et al.22) with slight modifications: after the EPSs or some inhibitors were dissolved in 10 μL of water at the given concentration, a 5-μL portion of hyaluronidase solution (MP Biomedicals, Santa Ana, CA, U.S.A.) that contained 4 mg of the enzyme in 1 mL of 100 mM sodium acetate buffer (pH 4.0) was added. .. After incubation of the mixture at 37°C for 20 min, a 10-μL portion of 0.5 mg/mL Compound 48/80 solution (MP Biomedicals) that contained 3.75 mg CaCl2·2H2O in 1 mL of the same buffer was added to the mixture and followed by the further incubation at 37°C for 20 min. A 25-μL portion of hyaluronic acid solution (Wako Pure Chemical Industries, Ltd.) that contained 0.8 mg in 1 mL of the same buffer was added and kept at 37°C for 40 min. To stop the reaction, after 10 μL of 400 mM NaOH was added to the mixture, the sample was further combined with 10 μL of 100 mM potassium borate buffer (pH 10.0), heated at 100°C for 3 min, and then immediately placed in an ice bath. .. A p-dimethylaminobenzaldehyde solution (p-DMAB, Wako Pure Chemical Industries, Ltd.) was prepared by diluting the 10× stock solution (5 g of p-DMAB, 6 mL of 10 M HCl, and 44 mL of acetate) with acetate.



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